Dr Amanda Nouwens
BSc Honours (Macquarie), PhD (Macquarie)
Senior Manager, Scientific Services
School of Chemistry and Molecular Biosciences
+61 7 334 69490
Molecular Biosciences Building (76), Room 429
Amanda coordinates and maintains the Mass Spectrometry (MS) facility. Provide research and teaching support to students and staff regarding MS across both biological and chemical applications (including identification/accurate mass, characterisation, quantitation). She provides instrument training and support for Circular Dichroism spectroscopy, contributes to and assists with funding applications to support the purchase of new equipment in the MS facility, as well as other general school scientific equipment.
Amanda supervises a number of scientific staff. She is a member of the Space and Facilities Management Committee and the School's representative on other ad hoc committees (eg FoS Research Infrastructure working group). She also oversees the equipment repair budget (EQREPS) and serves as fire warden for the Molecular Biosciences Building.
See our scientific facilities page for more information on instrumentation available in the facility.
Book Chapters
Fry, B. G., Undheim, E. A. B., Jackson, T. N. W., Georgieva, D., Vetter, I., Calvete, J. J., Schieb, H., Cribb, B. W., Yang, D. C., Daly, N. L., Manchadi, M. L. Roy, Gutierrez, J. M., Roelants, K., Lomonte, B., Nicholson, G. M., Dziemborowicz, S., Lavergne, V., Ragnarsson, L., Rash, L. D., Mobli, M., Hodgson, W. C., Casewell, N. R., Nouwens, A., Wagstaff, S. C., Ali, S. A., Whitehead, D. L., Herzig, V., Monagle, P., Kurniawan, N. D. ... Sunagar, K. (2015). Research methods. Venomous reptiles and their toxins: evolution, pathophysiology and biodiscovery. (pp. 153-214) New York, NY, United States: Oxford University Press.
Nouwens, Amanda and Mahler, Stephen (2012). Evolution of proteomic methods for analysis of complex biological samples - implications for personalized medicine.. Integrative Proteomics. (pp. 29-43) edited by Hon-Chiu Eastwood Leung, Tsz-Kwong Man and Ricardo J. Flores. Croatia: InTech. doi: 10.5772/29613
Journal Articles
Chaousis, Stephanie, Leusch, Frederic D. L., Nouwens, Amanda, Melvin, Steven D. and van de Merwe, Jason P. (2023). Influence of chemical dose and exposure duration on protein synthesis in green sea turtle primary cells. Journal of Proteomics, 285 104942, 1-9. doi: 10.1016/j.jprot.2023.104942
Dashevsky, Daniel, Baumann, Kate, Undheim, Eivind A. B., Nouwens, Amanda, Ikonomopoulou, Maria P., Schmidt, Justin O., Ge, Lilin, Kwok, Hang Fai, Rodriguez, Juanita and Fry, Bryan G. (2023). Functional and proteomic insights into Aculeata venoms. Toxins, 15 (3) 224, 1-25. doi: 10.3390/toxins15030224
Raza, Ali, Schulz, Benjamin L., Nouwens, Amanda, Naseem, Muhammad Noman, Kamran, Muhammad, Mantilla Valdivieso, Emily F., Kerr, Edward D., Constantinoiu, Constantin, Jonsson, Nicholas N., James, Peter and Tabor, Ala E. (2023). Application of quantitative proteomics to discover biomarkers for tick resistance in cattle. Frontiers in Immunology, 14 1091066, 1091066. doi: 10.3389/fimmu.2023.1091066
Chaousis, Stephanie, Leusch, Frederic DL., Limpus, Colin J., Nouwens, Amanda, Weijs, Liesbeth J., Weltmeyer, Antonia, Covaci, Adrian and van de Merwe, Jason P. (2023). Non-targeted proteomics reveals altered immune response in geographically distinct populations of green sea turtles (Chelonia mydas). Environmental Research, 216 (Pt 1) 114352, 1-9. doi: 10.1016/j.envres.2022.114352
Dashevsky, Daniel, Deuis, Jennifer R., Vetter, Irina, Huynh, Tam, Hodgson, Wayne C., Tan, Choo Hock, Nouwens, Amanda and Fry, Bryan G. (2021). Novel neurotoxic activity in Calliophis intestinalis venom. Neurotoxicity Research, 40 (1), 173-178. doi: 10.1007/s12640-021-00413-2
Rubbab, Tehseen, Pegg, Cassandra L., Phung, Toan K., Nouwens, Amanda S., Yeo, K.Y. Benjamin, Zacchi, Lucia F., Muhammad, Amna, Naqvi, S.M. Saqlan and Schulz, Benjamin L. (2021). N-glycosylation on Oryza sativa root germin-like protein 1 is conserved but not required for stability or activity. Biochemical and Biophysical Research Communications, 553, 72-77. doi: 10.1016/j.bbrc.2021.03.024
Raza, Ali, Schulz, Benjamin L., Nouwens, Amanda, Jackson, Lousie A., Piper, Emily K., James, Peter, Jonsson, Nicholas N. and Tabor, Ala E. (2021). Serum proteomes of Santa Gertrudis cattle before and after infestation with Rhipicephalus australis ticks. Parasite Immunology, 43 (7) e12836, e12836. doi: 10.1111/pim.12836
Dashevsky, Daniel, Rokyta, Darin, Frank, Nathaniel, Nouwens, Amanda and Fry, Bryan G. (2021). Electric blue: molecular evolution of three-finger toxins in the long-glanded coral snake species Calliophis bivirgatus. Toxins, 13 (2) 124, 1-16. doi: 10.3390/toxins13020124
Chaousis, Stephanie, Leusch, Frederic D.L., Nouwens, Amanda, Melvin, Steven D. and van de Merwe, Jason P. (2021). Changes in global protein expression in sea turtle cells exposed to common contaminants indicates new biomarkers of chemical exposure. Science of the Total Environment, 751 141680, 1-9. doi: 10.1016/j.scitotenv.2020.141680
Harris, Richard J., Youngman, Nicholas J., Zdenek, Christina N., Huynh, Tam M., Nouwens, Amanda, Hodgson, Wayne C., Harrich, David, Dunstan, Nathan, Portes-Junior, José A. and Fry, Bryan G. (2020). Assessing the binding of venoms from aquatic elapids to the nicotinic acetylcholine receptor orthosteric site of different prey models. International Journal of Molecular Sciences, 21 (19) 7377, 1-13. doi: 10.3390/ijms21197377
O’Brien, Caitlin A., Pegg, Cassandra L., Nouwens, Amanda S., Bielefeldt-Ohmann, Helle, Huang, Bixing, Warrilow, David, Harrison, Jessica J., Haniotis, John, Schulz, Benjamin L., Paramitha, Devina, Colmant, Agathe M. G., Newton, Natalee D., Doggett, Stephen L., Watterson, Daniel, Hobson-Peters, Jody and Hall, Roy A. (2020). A unique relative of Rotifer birnavirus Isolated from Australian mosquitoes. Viruses, 12 (9) 1056, 1056. doi: 10.3390/v12091056
Harris, Richard J., Zdenek, Christina N., Nouwens, Amanda, Sweeney, Charlotte, Dunstan, Nathan and Fry, Bryan G. (2020). A symmetry or asymmetry: functional and compositional comparison of venom from the left and right glands of the Indochinese spitting cobra (Naja siamensis). Toxicon: X, 7 100050, 100050. doi: 10.1016/j.toxcx.2020.100050
Zalucki, Yaramah M., Jen, Freda E.-C., Pegg, Cassandra L., Nouwens, Amanda S., Schulz, Benjamin L. and Jennings, Michael P. (2020). Evolution for improved secretion and fitness may be the selective pressures leading to the emergence of two NDM alleles. Biochemical and Biophysical Research Communications, 524 (3), 555-560. doi: 10.1016/j.bbrc.2020.01.135
Boe‐Hansen, Gry Brandt, Rêgo, João Paulo A., Satake, Nana, Venus, Bronwyn, Sadowski, Pawel, Nouwens, Amanda, Li, Yutao and McGowan, Michael (2020). Effects of increased scrotal temperature on semen quality and seminal plasma proteins in Brahman bulls. Molecular Reproduction and Development, 87 (5) mrd.23328, 574-597. doi: 10.1002/mrd.23328
Scheib, Holger, Nekaris, K. Anne-Isola, Rode-Margono, Johanna, Ragnarsson, Lotten, Baumann, Kate, Dobson, James S., Wirdateti, Wirdateti, Nouwens, Amanda, Nijman, Vincent, Martelli, Paolo, Ma, Rui, Lewis, Richard J., Kwok, Hang Fai and Fry, Bryan Grieg (2020). The toxicological intersection between allergen and toxin: a structural comparison of the cat dander allergenic protein Fel d1 and the slow loris brachial gland secretion protein. Toxins, 12 (2) toxins12020086, 86. doi: 10.3390/toxins12020086
Debono, Jordan, Dashevsky, Daniel, Nouwens, Amanda and Fry, Bryan G. (2020). The sweet side of venom: glycosylated prothrombin activating metalloproteases from Dispholidus typus (boomslang) and Thelotornis mossambicanus (twig snake). Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 227 108625, 108625. doi: 10.1016/j.cbpc.2019.108625
Gao, Shu-Hong, Ho, Jun Yuan, Fan, Lu, Nouwens, Amanda, Hoelzle, Robert D., Schulz, Benjamin, Guo, Jianhua, Zhou, Jizhong, Yuan, Zhiguo and Bond, Philip L. (2019). A comparative proteomic analysis of Desulfovibrio vulgaris Hildenborough in response to the antimicrobial agent free nitrous acid. Science of The Total Environment, 672, 625-633. doi: 10.1016/j.scitotenv.2019.03.442
Debono, Jordan, Bos, Mettine H.A., Nouwens, Amanda, Ge, Lilin, Frank, Nathaniel, Kwok, Hang Fai and Fry, Bryan (2019). Habu coagulotoxicity: clinical implications of the functional diversification of Protobothrops snake venoms upon blood clotting factors. Toxicology in Vitro, 55, 62-74. doi: 10.1016/j.tiv.2018.11.008
Garcia, Mario D., Chua, Sheena M. H., Low, Yu-Shang, Lee, Yu-Ting, Agnew-Francis, Kylie, Wang, Jian-Guo, Nouwens, Amanda, Lonhienne, Thierry, Williams, Craig M., Fraser, James A. and Guddat, Luke W. (2018). Commercial AHAS-inhibiting herbicides are promising drug leads for the treatment of human fungal pathogenic infections. Proceedings of the National Academy of Sciences of the United States of America, 115 (41), E9649-E9658. doi: 10.1073/pnas.1809422115
Kummari, Lalith K., Butler, Mark S., Furlong, Emily, Blundell, Ross, Nouwens, Amanda, Silva, Alberto B., Kappler, Ulrike, Fraser, James A., Kobe, Bostjan, Cooper, Matthew A. and Robertson, Avril A.B. (2018). Antifungal benzo[b]thiophene 1,1-dioxide IMPDH inhibitors exhibit pan-assay interference (PAINS) profiles. Bioorganic and Medicinal Chemistry, 26 (20), 5408-5419. doi: 10.1016/j.bmc.2018.09.004
Laloo, Andrew E., Wei, Justin, Wang, Dongbo, Narayanasamy, Shaman, Vanwonterghem, Inka, Waite, David, Steen, Jason, Kaysen, Anne, Heintz-Buschart, Anna, Wang, Qilin, Schulz, Benjamin, Nouwens, Amanda, Wilmes, Paul, Hugenholtz, Philip, Yuan, Zhiguo and Bond, Philip L. (2018). Mechanisms of persistence of the ammonia-oxidizing bacteria Nitrosomonas to the biocide free nitrous acid. Environmental science & technology, 52 (9), 5386-5397. doi: 10.1021/acs.est.7b04273
Karbanowicz, Thomas P., Nouwens, Amanda, Tabor, Ala E. and Rodriguez-Valle, Manuel (2018). Comparison of protein gut samples from rhipicephalus spp. using a crude and an innovative preparation method for proteome analysis. Veterinary Sciences, 5 (1) 30, 30. doi: 10.3390/vetsci5010030
Schulz, Benjamin L., Phung, Toan K., Bruschi, Michele, Janusz, Agnieszka, Stewart, Jeff, Meehan, John, Healy, Peter, Nouwens, Amanda S., Fox, Glen P. and Vickers, Claudia E. (2018). Process Proteomics of Beer Reveals a Dynamic Proteome with Extensive Modifications. Journal of Proteome Research, 17 (4), 1647-1653. doi: 10.1021/acs.jproteome.7b00907
Goldenberg, Jonathan, Cipriani, Vittoria, Jackson, Timothy N. W., Arbuckle, Kevin, Debono, Jordan, Dashevsky, Daniel, Panagides, Nadya, Ikonomopoulou, Maria P., Koludarov, Ivan, Li, Bin, Santana, Renan Castro, Nouwens, Amanda, Jones, Alun, Hay, Chris, Dunstan, Nathan, Allen, Luke, Bush, Brian, Miles, John J, Ge, Lilin, Kwok, Hang Fai and Fry, Bryan G (2018). Proteomic and functional variation within black snake venoms (Elapidae: Pseudechis). Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 205, 53-61. doi: 10.1016/j.cbpc.2018.01.001
Xu, Zhouwei, Lee, Aven, Nouwens, Amanda, David Henderson, Robert and Mccombe, Pamela Ann (2018). Mass spectrometry analysis of plasma from amyotrophic lateral sclerosis and control subjects. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, 19 (5-6), 1-15. doi: 10.1080/21678421.2018.1433689
Lonhienne, Thierry, Garcia, Mario D., Pierens, Gregory, Mobli, Mehdi, Nouwens, Amanda and Guddat, Luke W. (2018). Structural insights into the mechanism of inhibition of AHAS by herbicides. Proceedings of the National Academy of Sciences of the United States of America, 115 (9), E1945-E1954. doi: 10.1073/pnas.1714392115
Dashevsky, Daniel, Debono, Jordan, Rokyta, Darin, Nouwens, Amanda, Josh, Peter and Fry, Bryan G. (2018). Three-Finger Toxin Diversification in the Venoms of Cat-Eye Snakes (Colubridae: Boiga). Journal of Molecular Evolution, 86 (8), 531-545. doi: 10.1007/s00239-018-9864-6
Dobson, James, Yang, Daryl, Op den Brouw, Bianca, Cochran, Chip, Huynh, Tam, Kurrupu, Sanjaya, Sánchez, Elda E., Massey, Daniel J., Baumann, Kate, Jackson, Timothy N. W., Nouwens, Amanda, Josh, Peter, Neri-Castro, Edgar, Alagón, Alejandro, Hodgson, Wayne C. and Fry, Bryan G. (2017). Rattling the border wall: pathophysiological implications of functional and proteomic venom variation between Mexican and US subspecies of the desert rattlesnake Crotalus scutulatus. Comparative Biochemistry and Physiology. Part C: Toxicology and Pharmacology, 205, 62-69. doi: 10.1016/j.cbpc.2017.10.008
Tayeb, Hossam H, Piantavigna, Stefania, Howard, Christopher B, Nouwens, Amanda, Mahler, Stephen M, Middelberg, Anton P J, He, Lizhong, Holt, Stephen A and Sainsbury, Frank (2017). Insights into the interfacial structure-function of poly(ethylene glycol)-decorated peptide-stabilised nanoscale emulsions. Soft matter, 13 (43), 7953-7961. doi: 10.1039/c7sm01614j
Grobbler, Christy, Virdis, Bernardino, Nouwens, Amanda, Harnisch, Falk, Rabaey, Korneel and Bond, Philip L. (2017). Effect of the anode potential on the physiology and proteome of Shewanella oneidensis MR-1. Bioelectrochemistry, 119, 172-179. doi: 10.1016/j.bioelechem.2017.10.001
Koludarov, Ivan, Jackson, Timothy N. W., op den Brouw, Bianca, Dobson, James, Dashevsky, Daniel, Arbuckle, Kevin, Clemente, Christofer J., Stockdale, Edward J., Cochran, Chip, Debono, Jordan, Stephens, Carson, Panagides, Nadya, Li, Bin, Manchadi, Mary-Louise Roy, Violette, Aude, Fourmy, Rudy, Hendrikx, Iwan, Nouwens, Amanda, Clements, Judith, Martelli, Paolo, Kwok, Hang Fai and Fry, Bryan G. (2017). Enter the dragon: the dynamic and multifunctional evolution of anguimorpha lizard venoms. Toxins, 9 (8) 242, 242. doi: 10.3390/toxins9080242
Casewell, Nicholas R., Visser, Jeroen C., Baumann, Kate, Dobson, James, Han, Han, Kuruppu, Sanjaya, Morgan, Michael, Romilio, Anthony, Weisbecker, Vera, Mardon, Karine, Ali, Syed A., Debono, Jordan, Koludarov, Ivan, Que, Ivo, Bird, Gregory C., Cooke, Gavan M., Nouwens, Amanda, Hodgson, Wayne C., Wagstaff, Simon C., Cheney, Karen L., Vetter, Irina, van der Weerd, Louise, Richardson, Michael K. and Fry, Bryan G. (2017). The Evolution of Fangs, Venom, and Mimicry Systems in Blenny Fishes (vol 27, pg 1184, 2017). Current Biology, 27 (10), 1549-1550. doi: 10.1016/j.cub.2017.05.009
Debono, Jordan, Dobson, James, Casewell, Nicholas R., Romilio, Anthony, Li, Bin, Kurniawan, Nyoman, Mardon, Karine, Weisbecker, Vera, Nouwens, Amanda , Kwok, Hang Fai and Fry, Bryan G. (2017). Coagulating colubrids: evolutionary, pathophysiological and biodiscovery implications of venom variations between boomslang (Dispholidus typus) and twig snake (Thelotornis mossambicanus). Toxins, 9 (5) 171, 171. doi: 10.3390/toxins9050171
Santana, Renan C., Perez, David, Dobson, James, Panagides, Nadya, Raven, Robert J., Nouwens, Amanda, Jones, Alun, King, Glenn F. and Fry, Bryan G. (2017). Venom profiling of a population of the theraphosid spider Phlogius crassipes reveals continuous ontogenetic changes from juveniles through adulthood. Toxins, 9 (4) 116, 116. doi: 10.3390/toxins9040116
Casewell, Nicholas R., Visser, Jeroen C., Baumann, Kate, Dobson, James, Han, Han, Kuruppu, Sanjaya, Morgan, Michael, Romilio, Anthony, Weisbecker, Vera, Ali, Syed A., Debono, Jordan, Koludarov, Ivan, Que, Ivo, Bird, Gregory C., Cooke, Gavan M., Nouwens, Amanda, Hodgson, Wayne C., Wagstaff, Simon C., Cheney, Karen L., Vetter, Irina, van der Weerd, Louise, Richardson, Michael K. and Fry, Bryan G. (2017). The evolution of fangs, venom, and mimicry systems in blenny fishes. Current Biology, 27 (8), 1184-1191. doi: 10.1016/j.cub.2017.02.067
Sagulenko, Evgeny, Nouwens, Amanda, Webb, Richard I., Green, Kathryn, Yee, Benjamin, Morgan, Garry, Leis, Andrew, Lee, Kuo-Chang, Butler, Margaret K., Chia, Nicholas, Pham, Uyen Thi Phuong, Lindgreen, Stinus, Catchpole, Ryan, Poole, Anthony M. and Fuerst, John A. (2017). Nuclear pore-like structures in a compartmentalized bacterium. PLoS One, 12 (2) e0169432, e0169432. doi: 10.1371/journal.pone.0169432
Garcia, Mario D., Nouwens, Amanda, Lonhienne, Thierry G. and Guddat, Luke W. (2017). Comprehensive understanding of acetohydroxyacid synthase inhibition by different herbicide families. Proceedings of the National Academy of Sciences, 114 (7), E1091-E1100. doi: 10.1073/pnas.1616142114
Jackson, Timothy N. W., Koludarov, Ivan, Ali, Syed A., Dobson, James, Zdenek, Christina N., Dashevsky, Daniel, Op den Brouw, Bianca, Masci, Paul P., Nouwens, Amanda, Josh, Peter, Goldenberg, Jonathan, Cipriani, Vittoria, Hay, Chris, Hendrikx, Iwan, Dunstan, Nathan, Allen, Luke and Fry, Bryan G. (2016). Rapid radiations and the race to redundancy: an investigation of the evolution of Australian elapid snake venoms. Toxins, 8 (11), 309. doi: 10.3390/toxins8110309
Yang, Daryl C., Deuis, Jennifer R., Dashevsky, Daniel, Dobson, James, Jackson, Timothy N. W., Brust, Andreas, Xie, Bing, Koludarov, Ivan, Debono, Jordan, Hendrikx, Iwan, Hodgson, Wayne C., Josh, Peter, Nouwens, Amanda, Baillie, Gregory J., Bruxner, Timothy J. C., Alewood, Paul F., Peng Lim, Kelvin Kok, Frank, Nathaniel, Vetter, Irina and Fry, Bryan G. (2016). The snake with the scorpion's sting: novel three-finger toxin sodium channel activators from the venom of the long-glanded blue coral snake (Calliophis bivirgatus). Toxins, 8 (10) A034, 303. doi: 10.3390/toxins8100303
Yeo, K. Y. Benjamin, Chrysanthopoulos, Panangiotis K., Nouwens, Amanda S., Marcellin, Esteban and Schulz, Benjamin L. (2016). High performance targeted mass spectrometry with precision data independent acquisition reveals site-specific glycosylation macroheterogeneity. Analytical Biochemistry, 510, 106-113. doi: 10.1016/j.ab.2016.06.009
Lei, Chang, Zhou, Liang, Xu, Chun, Sun, Xiaoran, Nouwens, Amanda and Yu, Chengzhong (2016). Binder-free TiO2 monolith-packed pipette tips for the enrichment of phosphorylated peptides. Australian Journal of Chemistry, 69 (12), 1396-1401. doi: 10.1071/CH16443
Lei, Chang, Xu, Chun, Nouwens, Amanda and Yu, Chengzhong (2016). Ultrasensitive ELISA+ enhanced by dendritic mesoporous silica nanoparticles. Journal of Materials Chemistry B, 4 (29), 4975-4979. doi: 10.1039/c6tb01023g
Debono, Jordan, Cochran, Chip, Kuruppu, Sanjaya, Nouwens, Amanda, Rajapakse, Niwanthi W., Kawasaki, Minami, Wood, Kelly, Dobson, James, Baumann, Kate, Jouiaei, Mahdokht, Jackson, Timothy N. W., Koludarov, Ivan, Low, Dolyce, Ali, Syed A., Smith, A. Ian, Barnes, Andrew and Fry, Bryan G. (2016). Canopy venom: proteomic comparison among new world arboreal pit-viper venoms. Toxins, 8 (7) 210, 210.1-210.19. doi: 10.3390/toxins8070210
Du, Qianyun Sharon, Trabi, Manuela, Richards, Renee Stirling, Mirtschin, Peter, Madaras, Frank, Nouwens, Amanda, Zhao, Kong-Nan, De Jersey, John, Lavin, Martin F., Guddat, Luke W. and Masci, Paul P. (2016). Characterization and structural analysis of a potent anticoagulant phospholipase A2 from Pseudechis australis snake venom. Toxicon, 111, 37-49. doi: 10.1016/j.toxicon.2015.12.017
Lonhienne, Thierry, Nouwens, Amanda, Williams, Craig M., Fraser, James A., Lee, Yu-Ting, West, Nicholas P. and Guddat, Luke W. (2016). Commercial herbicides can trigger the oxidative inactivation of acetohydroxyacid synthase. Angewandte Chemie, 128 (13), 4319-4323. doi: 10.1002/ange.201511985
Lonhienne, Thierry, Nouwens, Amanda, Williams, Craig M., Fraser, James A., Lee, Yu-Ting, West, Nicholas P. and Guddat, Luke W. (2016). Commercial Herbicides Can Trigger the Oxidative Inactivation of Acetohydroxyacid Synthase. Angewandte Chemie - International Edition, 55 (13), 4247-4251. doi: 10.1002/anie.201511985
Boe-Hansen G.B., Rego J.P.A., Crisp, J.M., Moura, A.A., Nouwens, A.S., Li, Y., Venus, B., Burns, B.M. and McGowan, M.R. (2015). Seminal plasma proteins and their relationship with percentage of morphologically normal sperm in 2-year-old Brahman (Bos indicus) bulls. Animal Reproduction Science, 162, 20-30. doi: 10.1016/j.anireprosci.2015.09.003
Rego, J. P. A., Moura, A. A., Nouwens, A. S., McGowan, M. R. and Boe-Hansen, G. B. (2015). Seminal plasma protein profiles of ejaculates obtained by internal artificial vagina and electroejaculation in Brahman bulls.. Animal Reproduction Science, 160, 126-137. doi: 10.1016/j.anireprosci.2015.07.015
Chang, Rachel Yoon Kyung, Etheridge, Naomi, Nouwens, Amanda S. and Dodd, Peter R. (2015). SWATH analysis of the synaptic proteome in Alzheimer's disease. Neurochemistry International, 87, 1-12. doi: 10.1016/j.neuint.2015.04.004
Lei, Chang, Xu, Chun, Noonan, Owen, Meka, Anand Kumar, Zhang, Long, Nouwens, Amanda and Yu, Chengzhong (2015). Mesoporous materials modified by aptamers and hydrophobic groups assist ultra-sensitive insulin detection in serum. Chemical Communications, 51 (71), 13642-13645. doi: 10.1039/c5cc04458h
Grobbler, Christy, Virdis, Bernardino, Nouwens, Amanda, Harnisch, Falk, Rabaey, Korneel and Bond, Philip L. (2015). Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes. Systematic and Applied Microbiology, 38 (2), 135-139. doi: 10.1016/j.syapm.2014.11.007
Jouiaei, Mahdokht, Casewell, Nicholas R., Yanagihara, Angel A., Nouwens, Amanda, Cribb, Bronwen W., Whitehead, Darryl, Jackson, Timothy N. W., Ali, Syed A., Wagstaff, Simon C., Koludarov, Ivan, Alewood, Paul, Hansen, Jay and Fry, Bryan G. (2015). Firing the sting: chemically induced discharge of cnidae reveals novel proteins and peptides from box jellyfish (Chironex fleckeri) venom. Toxins, 7 (3), 936-950. doi: 10.3390/toxins7030936
Orellana, Camila A., Marcellin, Esteban, Schulz, Benjamin L., Nouwens, Amanda S., Gray, Peter P. and Nielsen, Lars K. (2015). High-antibody-producing Chinese hamster ovary cells up-regulate intracellular protein transport and glutathione synthesis. Journal of Proteome Research, 14 (2), 609-618. doi: 10.1021/pr501027c
Ali, Syed A., Jackson, Timothy N., Casewell, Nicholas R., Low, Dolyce H.W., Rossi, Sarah, Baumann, Kate, Fathinia, Behzad, Visser, Jeroen, Nouwens, Amanda, Hendrikx, Iwan, Jones, Alun and Fry, Bryan G. (2015). Extreme venom variation in Middle Eastern vipers: A proteomics comparison of Eristicophis macmahonii, Pseudocerastes fieldi and Pseudocerastes persicus. Journal of Proteomics, 116, 106-113. doi: 10.1016/j.jprot.2014.09.003
Koludarov, Ivan, Jackson, Timothy N. W., Sunagar, Kartik, Nouwens, Amanda, Hendrikx, Iwan and Fry, Bryan G. (2014). Fossilized venom: the unusually conserved venom profiles of heloderma species (beaded lizards and gila monsters).. Toxins, 6 (12), 3582-3595. doi: 10.3390/toxins6123582
Chang, Rachel Yoon Kyung, Etheridge, Naomi, Dodd, Peter R. and Nouwens, Amanda S. (2014). Targeted quantitative analysis of synaptic proteins in Alzheimer's disease brain. Neurochemistry International, 75, 66-75. doi: 10.1016/j.neuint.2014.05.011
Rego, J. P. A., Crisp, J. M., Moura, A. A., Nouwens, A. S., Li, Y., Venus, B., Corbet, N. J., Corbet, D. H., Burns, B. M., Boe-Hansen, G. B. and McGowan, M. R. (2014). Seminal plasma proteome of electroejaculated Bos indicus bulls. Animal Reproduction Science, 148 (1-2), 1-17. doi: 10.1016/j.anireprosci.2014.04.016
Lei, Chang, Noonan, Owen, Jambhrunkar, Siddharth, Qian, Kun, Xu, Chun, Zhang, Jun, Nouwens, Amanda and Yu, Chengzhong (2014). Sensitive detection of human insulin using a designed combined pore approach. Small, 10 (12), 2413-2418. doi: 10.1002/smll.201303748
Baumann, Kate, Casewell, Nicholas R., Ali, Syed A., Jackson, Timothy N. W., Vetter, Irina, Dobson, James S., Cutmore, Scott C., Nouwens, Amanda, Lavergne, Vincent and Fry, Bryan G. (2014). A ray of venom: Combined proteomic and transcriptomic investigation of fish venom composition using barb tissue from the blue-spotted stingray (Neotrygon kuhlii). Journal of Proteomics, 109, 188-198. doi: 10.1016/j.jprot.2014.06.004
Chang, Rachel Yoon Kyung, Etheridge, Naomi, Dodd, Peter and Nouwens, Amanda (2014). Quantitative multiple reaction monitoring analysis of synaptic proteins from human brain. Journal of Neuroscience Methods, 227, 189-210. doi: 10.1016/j.jneumeth.2014.02.016
Jesupret, Clémence, Baumann, Kate, Jackson, Timothy N. W., Ali, Syed Abid, Yang, Daryl C., Greisman, Laura, Kern, Larissa, Steuten, Jessica, Jouiaei, Mahdokht, Casewell, Nicholas R., Undheim, Eivind A. B., Koludarov, Ivan, Debono, Jordan, Low, Dolyce H. W., Rossi, Sarah, Panagides, Nadya, Winter, Kelly, Ignjatovic, Vera, Summerhayes, Robyn, Jones, Alun, Nouwens, Amanda, Dunstan, Nathan, Hodgson, Wayne C., Winkel, Kenneth D., Monagle, Paul and Fry, Bryan Grieg (2014). Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades. Journal of Proteomics, 105, 285-294. doi: 10.1016/j.jprot.2014.01.004
Kappler, Ulrike and Nouwens, Amanda S. (2013). Metabolic adaptation and trophic strategies of soil bacteria--C1- metabolism and sulfur chemolithotrophy in Starkeya novella. Frontiers in Microbiology, 4 (304), 1-12. doi: 10.3389/fmicb.2013.00304
Ali, Syed A., Baumann, Kate, Jackson, Timothy N. W., Wood, Kelly, Mason, Steven, Undheim, Eivind A.B., Nouwens, Amanda, Koludarov, Ivan, Hendrikx, Iwan, Jones, Alun and Fry, Bryan Grieg (2013). Proteomic comparison of Hypnale hypnale (Hump-Nosed Pit-Viper) and Calloselasma rhodostoma (Malayan Pit-Viper) venoms. Journal of Proteomics, 91, 338-343. doi: 10.1016/j.jprot.2013.07.020
Friend, Lexie R., Landsberg, Michael J., Nouwens, Amanda S., Wei, Ying, Rothnagel, Joseph A. and Smith, Ross (2013). Arginine methylation of hnRNP A2 does not directly govern its subcellular localization. PLoS One, 8 (9) e75669, e75669.1-e75669.12. doi: 10.1371/journal.pone.0075669
Chang, Rachel Yoon Kyung, Nouwens, Amanda S., Dodd, Peter R. and Etheridge, Naomi (2013). The synaptic proteome in Alzheimer's disease. Alzheimer's & Dementia, 9 (5), 499-511. doi: 10.1016/j.jalz.2012.04.009
Qian, Kun, Zhou, Liang, Liu, Jian, Yang, Jie, Xu, Hongyi, Yu, Meihua, Nouwens, Amanda, Zou, Jin, Monteiro, Michael J. and Yu, Chengzhong (2013). Laser engineered graphene paper for mass spectrometry imaging. Scientific Reports, 3 (1) 1415, 1415.1-1415.7. doi: 10.1038/srep01415
Kappler, Ulrike and Nouwens, Amanda S. (2013). The molybdoproteome of Starkeya novella - insights into the diversity and functions of molybdenum containing proteins in response to changing growth conditions. Metallomics, 5 (4), 325-334. doi: 10.1039/C2MT20230A
Lei, Chang, Qian, Kun, Noonan, Owen, Nouwens, Amanda and Yu, Chengzhong (2013). Applications of nanomaterials in mass spectrometry analysis. Nanoscale, 5 (24), 12033-12042. doi: 10.1039/c3nr04194h
Neave, Matthew J., Streten-Joyce, Claire, Nouwens, Amanda S., Glasby, Chris J., McGuinness, Keith A., Parry, David L. and Gibb, Karen S. (2012). The transcriptome and proteome are altered in marine polychaetes (Annelida) exposed to elevated metal levels. Journal of Proteomics, 75 (9), 2721-2735. doi: 10.1016/j.jprot.2012.03.031
Jurczyluk, Julie, Nouwens, Amanda S., Holien, Jessica K., Adams, Timothy E., Lovrecz, George O., Parker, Michael W., Cohen, Scott B. and Bryan, Tracy M. (2011). Direct involvement of the TEN domain at the active site of human telomerase. Nucleic Acids Research, 39 (5), 1774-1788. doi: 10.1093/nar/gkq1083
Matigian, N, Abrahamsen, G, Sutharsan, R, Cook, AL, Vitale, AM, Nouwens, A, Bellette, B, An, JY, Anderson, M, Beckhouse, AG, Bennebroek, M, Cecil, R, Chalk, AM, Cochrane, J, Fan, YJ, Feron, F, McCurdy, R, McGrath, JJ, Murrell, W, Perry, C, Raju, J, Ravishankar, S, Silburn, PA, Sutherland, GT, Mahler, S, Mellick, GD, Wood, SA, Sue, CM, Wells, CA and Mackay-Sim, A (2010). Disease-specific, neurosphere-derived cells as models for brain disorders. Disease Models and Mechanisms, 3 (11-12), 785-798. doi: 10.1242/dmm.005447
Lonhienne, Thierry G. A., Sagulenko, Evgeny, Webb, Richard I., Lee, Kuo-Chang, Franke, Josef, Devos, Damien P., Nouwens, Amanda, Carroll, Bernard J. and Fuerst, John A. (2010). Endocytosis-like protein uptake in the bacterium Gemmata obscuriglobus. Proceedings of the National Acadamy of Sciences of the United State of America, 107 (29), 12883-12888. doi: 10.1073/pnas.1001085107
Balmori Melian, Ezequiel, Hinzman, Edward, Nagasaki, Tomoko, Firth, Andrew E., Wills, Norma M., Nouwens, Amanda S., Blitvich, Bradley J., Leung, Jason, Funk, Anneke, Atkins, John F., Hall, Roy and Khromykh, Alexander A. (2010). NS1 ' of flaviviruses in the Japanese encephalitis virus serogroup is a product of ribosomal frameshifting and plays a role in viral neuroinvasiveness. Journal of Virology, 84 (3), 1641-1647. doi: 10.1128/JVI.01979-09
Cao, Ying, Huschtscha, Lily I., Nouwens, Amanda S., Pickett, Hilda A., Neumann, Axel A., Chang, Andy C-M., Toouli, Christian D., Bryan, Tracy M. and Reddel, Roger R. (2008). Amplification of telomerase reverse transcriptase gene in human mammary epithelial cells with limiting telomerase RNA expression levels. Cancer Research, 68 (9), 3115-3123. doi: 10.1158/0008-5472.CAN-07-6377
Nouwens, Amanda S., Beatson, Scott A., Whitchurch, Cynthia B., Walsh, Bradley. J., Schweizer, Herbert P., Mattick, John S. and Cordwell, Stuart J. (2003). Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1. Microbiology-sgm, 149 (5), 1311-1322. doi: 10.1099/mic.0.25967-0
Nouwens, A. S., Walsh, B. J. and Cordwell, S. J. (2003). Application of proteomics to Pseudomonas aeruginosa. Advances in Biochemical Engineering Biotechnology, 83, 117-140. doi: 10.1007/3-540-36459-5_5
Cordwell, SJ, Nouwens, AS and Walsh, BJ (2001). Comparative proteomics of bacterial pathogens. Proteomics, 1 (4), 461-472. doi: 10.1002/1615-9861(200104)1:4<461::AID-PROT461>3.0.CO;2-S
Molloy, MP, Herbert, BR, Slade, MB, Rabilloud, T, Nouwens, AS, Williams, KL and Gooley, AA (2000). Proteomic analysis of the Escherichia coli outer membrane. European Journal of Biochemistry, 267 (10), 2871-2881. doi: 10.1046/j.1432-1327.2000.01296.x
Cordwell, SJ, Nouwens, AS, Verrills, NM, Basseal, DJ and Walsh, BJ (2000). Subproteomics based upon protein cellular location and relative solubilities in conjunction with composite two-dimensional electrophoresis gels. Electrophoresis, 21 (6), 1094-1103. doi: 10.1002/(SICI)1522-2683(20000401)21:6<1094::AID-ELPS1094>3.3.CO;2-S
Cordwell, SJ, Nouwens, AS, Verrills, NM, McPherson, JC, Hains, PG, Van Dyk, DD and Walsh, BJ (1999). The microbial proteome database - an automated laboratory catalogue for monitoring protein expression in bacteria. Electrophoresis, 20 (18), 3580-3588. doi: 10.1002/(SICI)1522-2683(19991201)20:18<3580::AID-ELPS3580>3.0.CO;2-2
Davison, AD, Gillings, MR, Jardine, DR, Karuso, P, Nouwens, AS, French, JJ, Veal, DA and Altavilla, N (1999). Sphingomonas paucimobilis BPSI-3 mutant AN2 produces a red catabolite during biphenyl degradation. Journal of Industrial Microbiology & Biotechnology, 23 (4-5), 314-319. doi: 10.1038/sj.jim.2900743
Harrison, MJ, Nouwens, AS, Jardine, DR, Zachara, NE, Gooley, AA, Nevalainen, H and Packer, NH (1998). Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei. European Journal of Biochemistry, 256 (1), 119-127. doi: 10.1046/j.1432-1327.1998.2560119.x
Conference Papers
Grobbler, C., Virdis, B., Nouwens, A., Harnisch, F., Rabaey, K. and Bond, P. L. (2013). Effect of the anodic potential in the extracellular electron transfer capability of Shewanella oneidensis MR-1. MFC4: 4th International Microbial Fuel Cell Conference, Cairns, QLD, Australia, 1-4 September, 2013.
Chang, R., Etheridge, N., Nouwens, A. and Dodd, P. (2011). Synaptic Degeneration in Alzheimer's Disease. Australian Neuroscience Society Annual Meeting, Auckland, New Zealand, 31 January - 3 February 2011.
Friend, L., Hillman, K., Skarshewski, A., Al Mansoori, S., Ng, W. N., Nouwens, A., Smith, R. and Rothnegal, J. A. (2011). Identification and characterisation of putative bioactive peptides encoded by upstream open reading frames. ComBio 2011, Cairns, Australia, 25-29 September 2011.
Friend, Lexie, Hillman, K., Skarshewski, A., Wang, X-Q., Al Mansoori, S., Nouwens, A., Smith, R. and Rothnagel, J. (2011). The extracurricular activities of upstream open reading frames. The East Coast Protein Meeting (ECPM, 2011), Coffs Harbour, NSW, Australia, 15-17 July 2011.
Toki, N., Nouwens, A., Friend, L., Smith, R. and Rothnagel, J. (2011). A proteomic approach to the identification of zebrafish hnRNP A/B proteins. The East Coast Protein Meeting, Coffs Harbour, 15-17 July 2011.
Dodd, P. R., Chang, R., Etheridge, N. and Nouwens, A. (2011). The synaptic proteome in Alzheimer's disease. International Society of Neurochemistry/European Society for Neurochemistry Biennial Meeting (23rd, ISN/ESN, 2011), Athens, Greece, 28 August-1 September 2011. Oxford, United Kingdom: Wiley-Blackwell Publishing. doi: 10.1111/j.1471-4159.2011.07326.x
Friend, Lexie, Landsberg, Michael, Nouwens, Amanda, Rothnagel, J., Smith, R. and Toki, Naoko (2010). Mass spectrometric detection of differential methylation of hnRNP A2/B1 paralogs in neuronal cells and cell lines. Human Proteome World Congress (HUPO 2010), Darling Harbour Convention Centre, Sydney, Australia, 19 - 23 September 2010.
Moura, Arlindo A., Mayes, Jessica, Silva, Michelle M., Souza, Carlos E. A., Holroyd, Richard, Nouwens, Amanda, Venus, Bronwyn and McGowan, Michael (2010). Proteomics of seminal plasma from Bos indicus bulls raised in Queensland-Australia. SSR2010: 43rd Annual Meeting of the Society for the Study of Reproduction: The Intersection Between Genetics, Genomics, and Reproductive Biology, Milwaukee, WI, U.S.A., 30 July-3 August 2010. Madison, WI, U.S.A.: Society for the Study of Reproduction.
Punyadeera, C., Nouwens, A. S., Stokes, J. R., Yakubov, G. and Cooper-White, J. (2010). Elucidation of changes in human saliva protein profiles during resting and stimulated states. Human Proteome World Congress (HUPO 2010), Sydney, Australia, 19 - 23 September 2010.
Neave, Matthew, Gibb, K., Glasby, C., McGuinness, K., Nouwens, A., Parry, D. and Streten, C. (2009). An integrated approach to marine sediment assessment and polychaetes as biological indicators. SETAC North America 30th Annual Meeting, New Orleans, LA, U.S.A., 19-23 November 2009. Pensacola, FL, U.S.A.: Society of Environmental Toxicology and Chemistry (SETAC).
Nouwens, AS, Cordwell, SJ, Larsen, MR, Molloy, MP, Gillings, M, Willcox, MDP and Walsh, BJ (2000). Complementing genomics with proteomics: The membrane subproteome of Pseudomonas aeruginosa PAO1. 6th Annual Conference of the Australian-Electrophoresis-Society, Melbourne Australia, Oct 21-22, 1999. doi: 10.1002/1522-2683(200011)21:17<3797::AID-ELPS3797>3.0.CO;2-P
Data Collection
Lei, Chang, Xu, Chun, Noonan, Owen, Meka, Anand Kumar, Zhang, Long, Nouwens, Amanda and Yu, Chengzhong (2015). Developing sensitive detection nanotechnology to better identify insulin in serum. The Royal Society of Chemistry. (Dataset) doi: 10.14264/uql.2016.842