Dr Jobichen Chacko
Research Fellow – Structural Biology and Drug Design
School of Chemistry and Molecular Biosciences
Researcher biography
Jobichen Chacko is a structural biologist with extensive experience in protein structural biology, protein-ligand interaction, proteases and drug discovery. He completed his PhD from National University of Singapore in the area of structural and functional studies on bacterial protein secretion system. He is proficient in the areas of Cryo-Electron microscopy (Single Particle Analysis), protein structure analysis and X-ray crystallography. He has published more than 40 research articles in high level journals and has more than 700 citations. His research interests are in host-pathogen interaction, protein secretion system, snake venom toxins and proteases. Jobichen is a member of the editorial advisory board for FEBS-openbio journal and serves as a reviewer for various scientific journals.
Book Chapters
Choong, Yeu Khai, Srinivasan, Mahalashmi and Jobichen, Chacko (2022). Proteases as therapeutic targets: a structural biology perspective. Therapeutic protein targets for drug discovery and clinical evaluation: bio-crystallography and drug design. (pp. 103-121) edited by Velmurugan, D, Bhattacharjee, Atanu and Gayath, D. Singapore: World Scientific Publishing Co.. doi: 10.1142/9789811254796_0005
Jobichen, Chacko and Selvanarayanan, V. (2019). Role of plant volatiles, pest-resistant varieties and transgenics in tri-trophic interactions. Experimental techniques in host-plant resistance. (pp. 245-252) edited by Akshay Kumar Chakravarthy and Venkatesan Selvanarayanan. Singapore: Springer. doi: 10.1007/978-981-13-2652-3_25
Journal Articles
Manisha, Yadav, Srinivasan, Mahalashmi, Jobichen, Chacko, Rosenshine, Ilan and Sivaraman, J. (2024). Sensing for survival: specialised regulatory mechanisms of Type III secretion systems in Gram-negative pathogens. Biological Reviews, 99 (3), 837-863. doi: 10.1111/brv.13047
Jobichen, Chacko, Chong, Tan Ying, Rattinam, Rajesh, Basak, Sandip, Srinivasan, Mahalashmi, Choong, Yeu Khai, Pandey, Kannu Priya, Tran Bich Ngoc,, Shi, Jian, Angayarkanni, Jayaraman and Sivaraman, J. (2023). Bacterioferritin nanocage structures uncover the biomineralization process in ferritins. PNAS Nexus, 2 (7) pgad235, pgad235. doi: 10.1093/pnasnexus/pgad235
Rehka, T., Sharma, Deepti, Lin, Fu, Choong, Yeu Khai, Lim, Chinchin, Jobichen, Chacko and Zhang, Congqiang (2023). Structural understanding of fungal terpene synthases for the formation of linear or cyclic terpene products. ACS Catalysis, 13 (7), 4949-4959. doi: 10.1021/acscatal.2c05598
Li, Jianwei, Jobichen, Chacko, Machida, Satoru, Meng, Sun, Read, Randy J., Chen, Hongying, Jian, Shi, Yuan, Yuren Adam and Sivaraman, J. (2023). Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage. Plos Biology, 21 (3) e3002023, e3002023. doi: 10.1371/journal.pbio.3002023
Shankar, Srihari, Ramachandran, Sarath, Tulsian, Nikhil, Radhakrishnan, Sridhar, Jobichen, Chacko and Sivaraman, J. (2022). A novel allosteric site employs a conserved inhibition mechanism in human kidney-type glutaminase. The FEBS Journal, 290 (9), 2437-2448. doi: 10.1111/febs.16658
Amobonye, Ayodeji, Singh, Samayaditya, Mukherjee, Koel, Jobichen, Chacko, Qureshi, Insaf Ahmed and Pillai, Santhosh (2022). Structural and functional insights into fungal glutaminase using a computational approach. Process Biochemistry, 117, 76-89. doi: 10.1016/j.procbio.2022.03.019
Zwe, Ye Htut, Yadav, Manisha, Ten, Michelle Mei Zhen, Srinivasan, Mahalashmi, Jobichen, Chacko, Sivaraman, J. and Li, Dan (2022). Bacterial antagonism of Chromobacterium haemolyticum and characterization of its putative type VI secretion system. Research in Microbiology, 173 (3) 103918, 103918. doi: 10.1016/j.resmic.2021.103918
Gavor, Edem, Choong, Yeu Khai, Jobichen, Chacko, Mok, Yu Keung, Kini, R. Manjunatha and Sivaraman, J. (2021). Structure of Aedes aegypti carboxypeptidase B1-inhibitor complex uncover the disparity between mosquito and non-mosquito insect carboxypeptidase inhibition mechanism. Protein Science, 30 (12), 2445-2456. doi: 10.1002/pro.4212
Walvekar, Varsha Ashok, Ramesh, Karthik, Jobichen, Chacko, Kannan, Muthu, Sivaraman, J., Kini, R. Manjunatha and Mok, Yu Keung (2021). Crystal structure of Aedes aegypti trypsin inhibitor in complex with μ-plasmin reveals role for scaffold stability in Kazal-type serine protease inhibitor. Protein Science, 31 (2), 470-484. doi: 10.1002/pro.4245
Chan, JingRu, Qinqin, Fu, Jianwei, Li, Ying, Chen, Machida, Satoru, Wei, Chen, Yuan, Yuren Adam and Jobichen, Chacko (2021). Structural and mechanistic insight into stem-loop RNA processing by yeast Pichia stipitis Dicer. Protein Science, 30 (6), 1210-1220. doi: 10.1002/pro.4086
Chichili, Vishnu Priyanka Reddy, Chew, Ti Weng, Shankar, Srihari, Er, Shi Yin, Chin, Cheen Fei, Jobichen, Chacko, Pan, Catherine Qiurong, Zhou, Yiting, Yeong, Foong May, Low, Boon Chuan and Sivaraman, J. (2021). Structural basis for p50RhoGAP BCH domain-mediated regulation of Rho inactivation. National Academy of Sciences. Proceedings, 118 (21) e2014242118, 1-12. doi: 10.1073/pnas.2014242118
Jobichen, Chacko, Chong, Tan Ying, Ling, Tai Hui and Sivaraman, J. (2021). The autocatalytic cleavage domain is not required for the activity of scpc, a virulence protease from streptococcus pyogenes: a structural insight. Biochemistry, 60 (19), 1564-1568. doi: 10.1021/acs.biochem.1c00185
Aoki-Shioi, Narumi, Jobichen, Chacko, Sivaraman, J. and Kini, R. Manjunatha (2020). Unusual quaternary structure of a homodimeric synergistic-type toxin from mamba snake venom defines its molecular evolution. Biochemical Journal, 477 (20), 3951-3962. doi: 10.1042/BCJ20200529
Jobichen, Chacko, Prabhakar, Mahalakshmi Tirumuru, Loh, Su Ning and Sivaraman, J. (2020). Structural basis for the inhibition mechanism of ecotin against neutrophil elastase by targeting the active site and secondary binding site. Biochemistry, 59 (30), 2788-2795. doi: 10.1021/acs.biochem.0c00493
Chen, Ying, Chan, Jingru, Chen, Wei, Li, Jianwei, Sun, Meng, Kannan, Gayathiri Sathyamoorthy, Mok, Yu-Keung, Yuan, Yuren Adam and Jobichen, Chacko (2020). SYNCRIP, a new player in pri-let-7a processing. RNA, 26 (3), 290-305. doi: 10.1261/rna.072959.119
Singh, Ajit Kumar, Datta, Aritreyee, Jobichen, Chacko, Luan, Sheng and Vasudevan, Dileep (2020). AtFKBP53: a chimeric histone chaperone with functional nucleoplasmin and PPIase domains. Nucleic Acids Research, 48 (3), 1531-1550. doi: 10.1093/nar/gkz1153
Foo, Chun Shin, Jobichen, Chacko, Hassan-Puttaswamy, Varuna, Dekan, Zoltan, Tae, Han-Shen, Bertrand, Daniel, Adams, David J., Alewood, Paul F., Sivaraman, J., Nirthanan, Selvanayagam and Kini, R. Manjunatha (2020). Fulditoxin, representing a new class of dimeric snake toxins, defines novel pharmacology at nicotinic ACh receptors. British Journal of Pharmacology, 177 (8), 1822-1840. doi: 10.1111/bph.14954
Roy, Amrita, Sun Qingxiang,, Alex, Chapeaurouge, Rajagopalan, Nandhakishore, Jobichen, Chacko, Sivaraman, J. and Kini, R. Manjunatha (2019). Identification of a α-helical molten globule intermediate and structural characterization of β-cardiotoxin, an all β-sheet protein isolated from the venom of Ophiophagus hannah (king cobra). Protein Science, 28 (5), 952-963. doi: 10.1002/pro.3605
Noreen, Zobia, Jobichen, Chacko, Abbasi, Rashda, Seetharaman, Jayaraman, Sivaraman, J. and Bokhari, Habib (2018). Structural basis for the pathogenesis of Campylobacter jejuni Hcp1, a structural and effector protein of the Type VI Secretion System. FEBS Journal, 285 (21), 4060-4070. doi: 10.1111/febs.14650
Jobichen, Chacko, Tan, Ying Chong, Prabhakar, Mahalakshmi Tirumuru, Nayak, Digant, Biswas, Debabrata, Pannu, Navraj S., Hanski, Emanuel and Sivaraman, J. (2018). Structure of ScpC, a virulence protease from Streptococcus pyogenes, reveals the functional domains and maturation mechanism. Biochemical Journal, 475 (17), 2847-2860. doi: 10.1042/bcj20180145
Hameed, Umar F. Shahul, Sanislav, Oana, Lay, Sui T., Annesley, Sarah J., Jobichen, Chacko, Fisher, Paul R., Swaminathan, Kunchithapadam and Arold, Stefan T. (2018). Proteobacterial origin of protein arginine methylation and regulation of complex I assembly by MidA. Cell Reports, 24 (8), 1996-2004. doi: 10.1016/j.celrep.2018.07.075
Liu, Bee Hui, Jobichen, Chacko, Chia, C. S. Brian, Chan, Tim Hon Man, Tang, Jing Ping, Chung, Theodora X. Y., Li, Jia, Poulsen, Anders, Hung, Alvin W., Koh-Stenta, Xiaoying, Tan, Yaw Sing, Verma, Chandra S., Tan, Hong Kee, Wu, Chan-Shuo, Li, Feng, Hill, Jeffrey, Joy, Joma, Yang, Henry, Chai, Li, Sivaraman, J. and Tenen, Daniel G. (2018). Targeting cancer addiction for SALL4 by shifting its transcriptome with a pharmacologic peptide. National Academy of Sciences. Proceedings, 115 (30), E7119-E7128. doi: 10.1073/pnas.1801253115
Verma, Apoorva, Lin, Fu, Tan, Ying Chong, Hidayat, Muhammad Nur, Jobichen, Chacko, Fan, Hao and Sivaraman, J. (2018). Biophysical studies and modelling indicate the binding preference of TAZ WW domain for LATS1 PPxY motif. Biochemical and Biophysical Research Communications, 502 (3), 307-312. doi: 10.1016/j.bbrc.2018.05.127
Iyer, Janaki Krishnamoorthy, Koh, Cho Yeow, Kazimirova, Maria, Roller, Ladislav, Jobichen, Chacko, Swaminathan, Kunchithapadam, Mizuguchi, Jun, Iwanaga, Sadaaki, Nuttall, Patricia A., Chan, Mark Y. and Kini, R. Manjunatha (2017). Avathrin: a novel thrombin inhibitor derived from a multicopy precursor in the salivary glands of the ixodid tick, Amblyomma variegatum. The FASEB Journal, 31 (7), 2981-2995. doi: 10.1096/fj.201601216R
Barnwal, Bhaskar, Jobichen, Chacko, Girish, Vallerinteavide Mavelli, Foo, Chun Shin, Sivaraman, J. and Kini, R. Manjunatha (2016). Ringhalexin from Hemachatus haemachatus: A novel inhibitor of extrinsic tenase complex. Scientific Reports, 6 25935, 1-13. doi: 10.1038/srep25935
Ghode, Pramila, Jobichen, Chacko, Ramachandran, Sarath, Bifani, Pablo and Sivaraman, J. (2015). Structural basis of mapping the spontaneous mutations with 5-flurouracil in uracil phosphoribosyltransferase from Mycobacterium tuberculosis. Biochemical and Biophysical Research Communications, 467 (3), 577-582. doi: 10.1016/j.bbrc.2015.09.133
Mohanty, Soumya, Jobichen, Chacko, Chichili, Vishnu Priyanka Reddy, Velazquez-Campoy, Adrian, Low, Boon Chuan, Hogue, Christopher W. V. and Sivaraman, J. (2015). Structural basis for a unique ATP synthase core complex from Nanoarcheaum equitans. Journal of Biological Chemistry, 290 (45), 27280-27296. doi: 10.1074/jbc.M115.677492
Nguyen, Van Sang, Jobichen, Chacko, Tan, Kang Wei, Tan, Yih Wan, Chan, Siew Leong, Ramesh, Karthik, Yuan, Yongming, Hong, Yunhan, Seetharaman, Jayaraman, Leung, Ka Yin, Sivaraman, J. and Mok, Yu Keung (2015). Structure of AcrH-AopB chaperone-translocator complex reveals a role for membrane hairpins in type III secretion system translocon assembly. Structure, 23 (11), 2022-2031. doi: 10.1016/j.str.2015.08.014
Lim, Yan Ting, Jobichen, Chacko, Wong, Jocelyn, Limmathurotsakul, Direk, Li, Shaowei, Chen, Yahua, Raida, Manfred, Srinivasan, Nalini, MacAry, Paul Anthony, Sivaraman, J. and Gan, Yunn-Hwen (2015). Extended loop region of Hcp1 is critical for the assembly and function of type VI secretion system in Burkholderia pseudomallei. Scientific Reports, 5 8235, 1-10. doi: 10.1038/srep08235
Jobichen, Chacko and Swaminathan, Kunchithapadam (2014). Molecular replacement with a large number of molecules in the asymmetric unit. Acta Crystallographica Section F-Structural Biology Communications, 70 (9), 1296-1302. doi: 10.1107/S2053230X14014381
Padavannil, Abhilash, Jobichen, Chacko, Yang Qinghua,, Seetharaman, Jayaraman, Velazquez-Campoy, Adrian, Yang, Liu, Pan, Shen Q. and Sivaraman, J. (2014). Dimerization of VirD2 binding protein is essential for Agrobacterium induced tumor formation in plants. PLoS Pathogens, 10 (3) e1003948, 1-12. doi: 10.1371/journal.ppat.1003948
Padavannil, Abhilash, Jobichen, Chacko, Mills, Erez, Velazquez-Campoy, Adrian, Li, Mo, Leung, Ka Yin, Mok, Yu Keung, Rosenshine, Ilan and Sivaraman, J. (2013). Structure of GrlR-GrlA complex that prevents GrlA activation of virulence genes. Nature Communications, 4 2546, 1-10. doi: 10.1038/ncomms3546
Pieraccini, Stefano, Rendine, Stefano, Jobichen, Chacko, Domadia, Prerna, Sivaraman, J., Francescato, Pierangelo, Speranza, Giovanna and Sironi, Maurizio (2013). Computer aided design of FtsZ targeting oligopeptides. RSC Advances, 3 (6), 1739-1743. doi: 10.1039/c2ra21886k
Jobichen, Chacko, Pal, Kuntal and Swaminathan, Kunchithapadam (2012). Crystal structure of mouse RhoA:GTPcS complex in a centered lattice. Journal of Structural and Functional Genomics, 13 (4), 241-245. doi: 10.1007/s10969-012-9143-5
Girish, Vallerinteavide Mavelli, Kumar, Sundramurthy, Joseph, Lissa, Jobichen, Chacko, Kini, R. Manjunatha and Sivaraman, J. (2012). Identification and structural characterization of a new three-finger toxin Hemachatoxin from Hemachatus haemachatus venom. PLoS One, 7 (10) e48112, 1-9. doi: 10.1371/journal.pone.0048112
Tan, Kang Wei, Jobichen, Chacko, Ong, Tan Ching, Gao, Yun Feng, Tiong, Yuen Sung, Wong, Kang Ning, Chew, Fook Tim, Sivaraman, J. and Mok, Yu Keung (2012). Crystal structure of Der f 7, a dust mite allergen from Dermatophagoides farinae. PLoS One, 7 (9) e44850, 1-8. doi: 10.1371/journal.pone.0044850
Jobichen, Chacko, Chakraborty, Smarajit, Li, Mo, Zheng, Jun, Joseph, Lissa, Mok, Yu-Keung, Leung, Ka Yin and Sivaraman, J. (2010). Structural basis for the secretion of EvpC: A key type VI secretion system protein from Edwardsiella tarda. PLoS One, 5 (9) e12910, 1-10. doi: 10.1371/journal.pone.0012910
Jobichen, Chacko, Fernandis, Aaron Z., Velazquez-Campoy, Adrian, Leung, Ka Yin, Mok, Yu-Keung, Wenk, Markus R. and Sivaraman, J. (2009). Identification and characterization of the lipid-binding property of GrIR, a locus of enterocyte effacement regulator. Biochemical Journal, 420, 191-199. doi: 10.1042/BJ20081588
Banerjee, Yajnavalka, Kumar, Sundramurthy, Jobichen, Chacko and Kini, R. Manjunatha (2007). Crystallization and preliminary X-ray diffraction analysis of hemextin A: a unique anticoagulant protein from Hemachatus haemachatus venom. Acta Crystallographica Section F-Structural Biology Communications, 63 (8), 701-703. doi: 10.1107/S1744309107034239
Jobichen, Chacko, Li, Mo, Yerushalmi, Gal, Tan, Yih Wan, Mok, Yu-Keung, Rosenshine, Ilan, Leung, Ka Yin and Sivaraman, J. (2007). Structure of GrIR and the implication of its EDED motif in mediating the regulation of type III secretion system in EHEC. PLoS Pathogens, 3 (5) e69, 639-646. doi: 10.1371/journal.ppat.0030069
Mohapatra, Purusottam, Chacko, Jobichen and Narayanasamy, P. (2006). Biological characteristics of certain Diamondback moth ecotypes. Journal of Entomology, 3 (4), 277-280. doi: 10.3923/je.2006.277.280
Preprints
Shin, Jeeeun, Jin, Ruitao, Gall, Barnabas, Jobichen, Chacko, Jackson, Colin, Rug, Melanie, Corry, Ben and Brock, Joseph (2024). Structure, dynamics and evolution of the Candida albicans multi-drug resistance ABC transporter CDR1. doi: 10.1101/2024.10.28.620768