A precision mode of optical microscopy in which a focused laser beam at a defined wavelength is scanned laterally along the x and y axes of a specimen in a raster pattern.

The emitted fluorescence is captured by a photomultiplier tube and displayed in pixels on a computer monitor. The pixel display dimensions are determined by the sampling rate of the electronics and the dimensions of the raster.

Single photons that are emitted away from the focal plane are blocked by a pinhole aperture located in a plane confocal with the specimen.

This technique enables the specimen to be optically sectioned along the z axis. As a result, optical slices of the specimen are imaged with high contrast and high resolution in x, y and z.

Contact us

Contact Dr Steven Mason for more information and bookings.

Applications

  • Multitrack imaging
  • Optical sectioning and 3D image manipulation
  • Time series and photo-bleaching
  • Spectral analysis and emission fingerprinting
  • Co-localisation studies
  • T-PMT imaging (laser as light source for transmitted light to PMT)
  • Tile and positional scanning
  • Live cell imaging
  • Reflectance scanning

Equipment and location

Equipment

ZEISS LSM 510 META laser scanning confocal microscope

  • The system is based on an upright Axioplan 2 imaging research class optical microscope
  • Laser lines available are 405, 458, 477, 488, 514, 543 and 633 nm
  • Imaging system comprises two PMTs and one META detector
  • Manual XY stage with motorized Z-drive
  • HR Colour Digital Imaging (Axiocam HRc) in VIS mode
  • Imaging software is ZEN 2009
  • Axiocam imaging software is Zen 2 (separate PC)

ZEISS LSM 510 inverted META laser scanning confocal microscope

  • The system is based on the Axio-Observer Z1 inverted research class optical microscope
  • Laser lines available are 405, 458, 477, 488, 514, 561 and 633 nm
  • Imaging system comprises two PMTs and one META detector
  • Motorised XY scanning stage with motorized Z-drive
  • HR Greyscale Digital Imaging (AxioCam MRm) in VIS mode
  • Imaging software is ZEN 2009
  • Axiocam MRm imaging software is ZEN 2012 (separate PC)

ZEISS LSM 710 inverted laser scanning confocal microscope

  • The system is based on the Axio-Observer Z1 inverted research class optical microscope
  • Laser lines available are 405, 458, 488, 514, 561 and 633nm
  • Imaging system comprises a 3-channel QUASAR spectral PMT array, consisting of 3x individual PMTs
  • Motorised XY scanning stage with motorized Z-drive and stage top frame Z PIEZO
  • Clear Perspex incubation chamber with temperature and CO2 control (for live cell imaging)
  • Imaging software is ZEN2.3

Software

  • Our associated image and data analysis software includes Imaris 9.2, ZEN deconvolution and Intellesis
  • An online NAS drive is available for storage of acquired images

Location

  • Confocal microscope systems: Molecular Biosciences Building (Building 76), Room 160
  • Computer workstations: Molecular Biosciences Building (Building 76), Room 111

Sample requirements

For optimal imaging resolution and image quality, you'll need to prepare samples based on the type of sample and imaging surface used. You'll need to discuss this with the Facility Manager.

The LSM710 inverted requires a 170um coverslip or plate/dish thickness for all objectives.

The LSM510 systems require 170um for oil/water immersion objectives.

The LSM510 Inverted has additional long focal distance objectives available.

The LSM510 Upright has a water dipping objective available.

Charges and considerations

Charges

Consultation is free.

External users: fees by negotiation with the Facility Manager.

SCMB and UQ users: contact the Facility Manager for fee details.

Considerations

UQ users have priority of access. 

You'll need to undertake the relevant building and other inductions before using equipment.

Users require a UQ username to access the booking calendar via public folders in Microsoft Outlook.