Mammalian tissue culture is the process of growing cells separated from the original organism.

The process is conducted within a sterile and controlled environment. The cells are grown within a culture medium, which contains the essential nutrients and energy sources necessary for the cells' survival and growth.

Tissue culture commonly refers to the culture of animal cells which may be primary or secondary (immortalised) in origin.

Contact us

Contact Dr Steven Mason for more information and bookings.

Applications

Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in-vitro model of the tissue in a defined and controlled environment that can be easily manipulated and analysed.

Applications include the study of:

  • cancer
  • immune system and brain cells
  • effects of growth factors and therapeutic agents
  • interactions and responses to chemicals, viruses and bacterium.

Equipment and location

Equipment

Our equipment and instruments include:

  • two Class II biological safety cabinets
  • three 170L incubators for routine tissue culture work
  • 200L incubator with UV-sterilised humidity pan and IR CO2 for more critical tissue culture applications. All incubators are fitted with 8mm laminated glass shelving to minimise vibrational artefacts
  • Nikon TS100 inverted bright-field and epi-fluorescent microscope with imaging system
  • Nikon Ts2 inverted bright-field (phase contrast) microscope with imaging system
  • Sigma bench top centrifuge for 15ml and 50ml falcon tubes
  • two Integra VACUSIP vacuum-based aspiration systems for tissue culture vessel evacuation
  • waste containers
  • 70 per cent ethanol and 12.5 per cent sodium hypochlorite solution.

Location

Molecular Biosciences Building (Building 76), Room 414

Related links

Microscopy and imaging

Charges and considerations

School researchers and students can use the equipment free of charge.

We offer training in tissue culture techniques for new users.

We provide a general tissue culture risk assessment for reference (2786), but you'll need to prepare your own risk assessment.

You'll need to undertake the relevant building and other inductions before using equipment. 

You'll also need to adhere to PC2 requirements and follow our operational procedures, which include completing facility log book entries and incubator content sheets, and OGTR paperwork.